Methods A Dot-ELISA method was used to determine the specific antibodies of envelope antigen, O antigen and H d antigen in Salmonella. Then the results were compared with the results of Widal's reaction. 方法采用Dot-ELISA测定伤寒沙门菌包膜抗原V、菌体抗原O和鞭毛抗原Hd的特异性抗体,并与肥达氏反应相比较。
Result The sera from 21 patients with typhoid fever were detected, 8 samples showed posi-tive by Widal's reaction, but 21 were positive by immunoblot assay. 结果:共检测确诊早期伤寒病人血清21例,肥达氏反应Hd阳性8例,免疫印迹酶联试验Hd阳性21例。
Dot-ELISA was ten times more sensitive than Widal's reaction, moreover, it had no cross-reaction. Dot-ELISA法比肥达氏反应敏感10倍以上,且无交叉反应。